JNK Kit ELISA

N° du produit ABIN4886162

Détail du produit JNK Kit ELISA

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Épitope: pThr183, pTyr185, total

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA Kit. This assay semi-quantitatively measures phophorylated JNK (Thr183/Tyr185) and Total JNK in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human/Mouse/Rat Phospho-JNK (pThr183/Tyr185) and total (pan) JNK.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Références pour JNK Kit ELISA (ABIN4886162)

Al-Gayyar, Al Youssef, Sherif, Shams, Abbas: "Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: effects on cytokine balance and apoptosis." dans: Life sciences, Vol. 111, Issue 1-2, pp. 18-26, (2014) (PubMed).

Détail du antigène

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Autre désignation: JNK (MAPK8 Produits)

Synonymes: JNK Kit ELISA, JNK-46 Kit ELISA, JNK1 Kit ELISA, JNK1A2 Kit ELISA, JNK21B1/2 Kit ELISA, PRKM8 Kit ELISA, SAPK1 Kit ELISA, SAPK1c Kit ELISA, AI849689 Kit ELISA, Prkm8 Kit ELISA, BSK Kit ELISA, BSK/DJNK Kit ELISA, Bsk Kit ELISA, CG5680 Kit ELISA, D-JNK Kit ELISA, D-junk Kit ELISA, DBSK/JNK Kit ELISA, DJNK Kit ELISA, DJNK/bsk Kit ELISA, Dmel\\CG5680 Kit ELISA, JNK/SAPK Kit ELISA, Jnk Kit ELISA, Junk Kit ELISA, SAPKa Kit ELISA, c-Jun Kit ELISA, dJNK Kit ELISA, jnk Kit ELISA, T10F20.15 Kit ELISA, jnk1 Kit ELISA, sapk1 Kit ELISA, mapk8 Kit ELISA, zgc:112379 Kit ELISA, JNKb Kit ELISA, mapk8b Kit ELISA, mitogen-activated protein kinase 8 Kit ELISA, Stress-activated protein kinase jnk-1 Kit ELISA, basket Kit ELISA, Protein kinase superfamily protein Kit ELISA, mitogen-activated protein kinase 8 L homeolog Kit ELISA, mitogen-activated protein kinase 8b Kit ELISA, mitogen-activated protein kinase 8B-like Kit ELISA, MAPK8 Kit ELISA, jnk-1 Kit ELISA, Mapk8 Kit ELISA, bsk Kit ELISA, ATMPK8 Kit ELISA, mapk8.L Kit ELISA, mapk8b Kit ELISA, LOC109094263 Kit ELISA

ID gène: 5599

UniProt: P45983

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins