JNK Kit ELISA

N° du produit ABIN6730536

Détail du produit JNK Kit ELISA

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse JNK1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse JNK1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Autre désignation: JNK1 (MAPK8 Produits)

Synonymes: JNK Kit ELISA, JNK-46 Kit ELISA, JNK1 Kit ELISA, JNK1A2 Kit ELISA, JNK21B1/2 Kit ELISA, PRKM8 Kit ELISA, SAPK1 Kit ELISA, SAPK1c Kit ELISA, AI849689 Kit ELISA, Prkm8 Kit ELISA, BSK Kit ELISA, BSK/DJNK Kit ELISA, Bsk Kit ELISA, CG5680 Kit ELISA, D-JNK Kit ELISA, D-junk Kit ELISA, DBSK/JNK Kit ELISA, DJNK Kit ELISA, DJNK/bsk Kit ELISA, Dmel\\CG5680 Kit ELISA, JNK/SAPK Kit ELISA, Jnk Kit ELISA, Junk Kit ELISA, SAPKa Kit ELISA, c-Jun Kit ELISA, dJNK Kit ELISA, jnk Kit ELISA, T10F20.15 Kit ELISA, jnk1 Kit ELISA, sapk1 Kit ELISA, mapk8 Kit ELISA, zgc:112379 Kit ELISA, JNKb Kit ELISA, mapk8b Kit ELISA, mitogen-activated protein kinase 8 Kit ELISA, Stress-activated protein kinase jnk-1 Kit ELISA, basket Kit ELISA, Protein kinase superfamily protein Kit ELISA, mitogen-activated protein kinase 8 L homeolog Kit ELISA, mitogen-activated protein kinase 8b Kit ELISA, mitogen-activated protein kinase 8B-like Kit ELISA, MAPK8 Kit ELISA, jnk-1 Kit ELISA, Mapk8 Kit ELISA, bsk Kit ELISA, ATMPK8 Kit ELISA, mapk8.L Kit ELISA, mapk8b Kit ELISA, LOC109094263 Kit ELISA

UniProt: A3KBF5

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins