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CXCL1 Kit ELISA
CXCL1
Reactivité: Souris, Chien
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4881492
Détail du produit CXCL1 Kit ELISA
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Antigène
Voir toutes CXCL1 Kits ELISA
CXCL1
(Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
Reactivité
Toutes les réactivités sur CXCL1 Kits ELISA
Souris, Chien
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Canine GRO alpha/CXCL1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Canine GRO alpha.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CXCL1 Kits ELISA
CXCL1
(Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
Autre désignation
CXCL1 (CXCL1 Produits )
Synonymes
FSP Kit ELISA, GRO1 Kit ELISA, GROa Kit ELISA, MGSA Kit ELISA, MGSA-a Kit ELISA, NAP-3 Kit ELISA, SCYB1 Kit ELISA, CINC-1 Kit ELISA, Gro1 Kit ELISA, CXCL1 Kit ELISA, Sdf1 Kit ELISA, Fsp Kit ELISA, KC Kit ELISA, Mgsa Kit ELISA, N51 Kit ELISA, Scyb1 Kit ELISA, gro Kit ELISA, GRO Kit ELISA, Gro Kit ELISA, C-X-C motif chemokine ligand 1 Kit ELISA, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) Kit ELISA, C-X-C motif chemokine ligand 12 Kit ELISA, chemokine (C-X-C motif) ligand 1 Kit ELISA, CXCL1 Kit ELISA, Cxcl1 Kit ELISA, Cxcl12 Kit ELISA, GRO1 Kit ELISA
Pathways
Autophagy
Vus récemment
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