TRKA Kit ELISA

N° du produit ABIN4884901

Détail du produit TRKA Kit ELISA

Antigène: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2-500 ng/mL

Seuil minimal de détection: 2 ng/mL

Application: ELISA

Fonction: Human TrkA ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human TrkA. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Autre désignation: TrkA (NTRK1 Produits)

Synonymes: MTC Kit ELISA, TRK Kit ELISA, TRK1 Kit ELISA, TRKA Kit ELISA, Trk-A Kit ELISA, p140-TrkA Kit ELISA, CDHF12 Kit ELISA, CDHR16 Kit ELISA, HSCR1 Kit ELISA, MEN2A Kit ELISA, MEN2B Kit ELISA, MTC1 Kit ELISA, PTC Kit ELISA, RET-ELE1 Kit ELISA, RET51 Kit ELISA, Trk Kit ELISA, C80751 Kit ELISA, Tkr Kit ELISA, TrkA Kit ELISA, trk Kit ELISA, trka Kit ELISA, CTRKA Kit ELISA, trkA Kit ELISA, neurotrophic receptor tyrosine kinase 1 Kit ELISA, ret proto-oncogene Kit ELISA, neurotrophic tyrosine kinase, receptor, type 1 Kit ELISA, NTRK1 Kit ELISA, RET Kit ELISA, Ntrk1 Kit ELISA, ntrk1 Kit ELISA

ID gène: 4914

UniProt: P04629

Pathways: Signalisation RTK, Neurotrophin Signaling Pathway, cAMP Metabolic Process