TNFRSF1A Kit ELISA

N° du produit ABIN4885363

Détail du produit TNFRSF1A Kit ELISA

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human TNF RI (TNFRSF1A) SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: TNF RI (TNFRSF1A Produits)

Synonymes: CD120a Kit ELISA, FPF Kit ELISA, MS5 Kit ELISA, TBP1 Kit ELISA, TNF-R Kit ELISA, TNF-R-I Kit ELISA, TNF-R55 Kit ELISA, TNFAR Kit ELISA, TNFR1 Kit ELISA, TNFR1-d2 Kit ELISA, TNFR55 Kit ELISA, TNFR60 Kit ELISA, p55 Kit ELISA, p55-R Kit ELISA, p60 Kit ELISA, TNF-R1 Kit ELISA, TNF-alphaR1 Kit ELISA, TNFRI Kit ELISA, TNFRp55 Kit ELISA, TNFalpha-R1 Kit ELISA, Tnfr-2 Kit ELISA, Tnfr1 Kit ELISA, fpf Kit ELISA, tbp1 Kit ELISA, p55-r Kit ELISA, tnf-r Kit ELISA, tnfar Kit ELISA, tnfr1 Kit ELISA, cd120a Kit ELISA, tnfr55 Kit ELISA, tnfr60 Kit ELISA, xtnfr1 Kit ELISA, wu:fj65b10 Kit ELISA, zgc:85914 Kit ELISA, TNFR I Kit ELISA, TNFRSF1A Kit ELISA, TNFA Kit ELISA, cTNF Kit ELISA, TNF receptor superfamily member 1A Kit ELISA, tumor necrosis factor receptor superfamily, member 1a Kit ELISA, TNF receptor superfamily member 1A S homeolog Kit ELISA, tumor necrosis factor Kit ELISA, TNFRSF1A Kit ELISA, Tnfrsf1a Kit ELISA, tnfrsf1a.S Kit ELISA, tnfrsf1a Kit ELISA, TNF Kit ELISA

ID gène: 7132

UniProt: P19438

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway