ACVRL1 Kit ELISA
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- Antigène Voir toutes ACVRL1 Kits ELISA
- ACVRL1 (Activin A Receptor Type II-Like 1 (ACVRL1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Activin Receptor Like Kinase 1 (ALK1). No significant cross-reactivity or interference between Activin Receptor Like Kinase 1 (ALK1) and analogues was observed.
- Sensibilité
- 0.056 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin Receptor Like Kinase 1 (ALK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin Receptor Like Kinase 1 (ALK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin Receptor Like Kinase 1 (ALK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin Receptor Like Kinase 1 (ALK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin Receptor Like Kinase 1 (ALK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin Receptor Like Kinase 1 (ALK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes ACVRL1 Kits ELISA
- ACVRL1 (Activin A Receptor Type II-Like 1 (ACVRL1))
- Autre désignation
- Activin Receptor Like Kinase 1 (ACVRL1 Produits)
- Synonymes
- ACVRL1 Kit ELISA, vgb Kit ELISA, alk1 Kit ELISA, zehn1109 Kit ELISA, hm:zehn1109 Kit ELISA, ACVRLK1 Kit ELISA, ALK-1 Kit ELISA, ALK1 Kit ELISA, HHT Kit ELISA, HHT2 Kit ELISA, ORW2 Kit ELISA, SKR3 Kit ELISA, TSR-I Kit ELISA, R-3 Kit ELISA, R3 Kit ELISA, SETHKIR Kit ELISA, AI115505 Kit ELISA, AI427544 Kit ELISA, Acvrlk1 Kit ELISA, Alk1 Kit ELISA, activin A receptor like type 1 Kit ELISA, activin A receptor type II-like 1 Kit ELISA, activin A receptor, type II-like 1 Kit ELISA, ACVRL1 Kit ELISA, acvrl1 Kit ELISA, Acvrl1 Kit ELISA
- Sujet
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Gene Name: Activin Receptor Like Kinase 1
Gene Aliases: ACVRL1, ACVRLK1, HHT2, ORW2, SKR3, TSR-I, TGF-B superfamily receptor type I, Activin A Receptor Type II-Like 1, Serine/Threonine-Protein Kinase Receptor R3
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