TNFRSF1A Kit ELISA

N° du produit ABIN5659671

Détail du produit TNFRSF1A Kit ELISA

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Autre désignation: Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A Produits)

Synonymes: CD120a Kit ELISA, FPF Kit ELISA, MS5 Kit ELISA, TBP1 Kit ELISA, TNF-R Kit ELISA, TNF-R-I Kit ELISA, TNF-R55 Kit ELISA, TNFAR Kit ELISA, TNFR1 Kit ELISA, TNFR1-d2 Kit ELISA, TNFR55 Kit ELISA, TNFR60 Kit ELISA, p55 Kit ELISA, p55-R Kit ELISA, p60 Kit ELISA, TNF-R1 Kit ELISA, TNF-alphaR1 Kit ELISA, TNFRI Kit ELISA, TNFRp55 Kit ELISA, TNFalpha-R1 Kit ELISA, Tnfr-2 Kit ELISA, Tnfr1 Kit ELISA, fpf Kit ELISA, tbp1 Kit ELISA, p55-r Kit ELISA, tnf-r Kit ELISA, tnfar Kit ELISA, tnfr1 Kit ELISA, cd120a Kit ELISA, tnfr55 Kit ELISA, tnfr60 Kit ELISA, xtnfr1 Kit ELISA, wu:fj65b10 Kit ELISA, zgc:85914 Kit ELISA, TNFR I Kit ELISA, TNFRSF1A Kit ELISA, TNFA Kit ELISA, cTNF Kit ELISA, TNF receptor superfamily member 1A Kit ELISA, tumor necrosis factor receptor superfamily, member 1a Kit ELISA, TNF receptor superfamily member 1A S homeolog Kit ELISA, tumor necrosis factor Kit ELISA, TNFRSF1A Kit ELISA, Tnfrsf1a Kit ELISA, tnfrsf1a.S Kit ELISA, tnfrsf1a Kit ELISA, TNF Kit ELISA

Sujet:

Gene Name: Tumor Necrosis Factor Receptor Superfamily, Member 1A

Gene Aliases: CD120A, P55, TBP1, FPF, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, P55-R, P60, Tumor necrosis factor receptor 1, Tumor necrosis factor-binding protein 1

ID gène: 25625

UniProt: P22934

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway