TRAIL Kit ELISA
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- Antigène Voir toutes TRAIL (TNFSF10) Kits ELISA
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). No significant cross-reactivity or interference between Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and analogues was observed.
- Sensibilité
- 6.2 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes TRAIL (TNFSF10) Kits ELISA
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Autre désignation
- Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TNFSF10 Produits)
- Synonymes
- APO2L Kit ELISA, Apo-2L Kit ELISA, CD253 Kit ELISA, TL2 Kit ELISA, TRAIL Kit ELISA, A330042I21Rik Kit ELISA, AI448571 Kit ELISA, APO-2L Kit ELISA, Ly81 Kit ELISA, Trail Kit ELISA, tl2 Kit ELISA, apo2l Kit ELISA, cd253 Kit ELISA, trail Kit ELISA, apo-2l Kit ELISA, trail1 Kit ELISA, xtrail1 Kit ELISA, tnfsf10l2 Kit ELISA, zgc:92320 Kit ELISA, TNLG6A Kit ELISA, TNF superfamily member 10 Kit ELISA, tumor necrosis factor (ligand) superfamily, member 10 Kit ELISA, tumor necrosis factor superfamily member 10 Kit ELISA, TNF superfamily member 10 S homeolog Kit ELISA, TNFSF10 Kit ELISA, Tnfsf10 Kit ELISA, tnfsf10.S Kit ELISA, tnfsf10 Kit ELISA
- Sujet
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Gene Name: Tumor Necrosis Factor Related Apoptosis Inducing Ligand
Gene Aliases: CD253, TNFSF10, APO2L, Apo2-L, TL2, TRAIL, Tumor Necrosis Factor Ligand Superfamily Member 10, Apo-2 Ligand
- ID gène
- 22035
- UniProt
- P50592
- Pathways
- Apoptose, Positive Regulation of Endopeptidase Activity
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