S100A8 Kit ELISA

N° du produit ABIN6951104

Détail du produit S100A8 Kit ELISA

Antigène: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse S100 A8 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Autre désignation: S100 A8 (S100A8 Produits)

Synonymes: 60B8AG Kit ELISA, CAGA Kit ELISA, CFAG Kit ELISA, CGLA Kit ELISA, CP-10 Kit ELISA, L1Ag Kit ELISA, MA387 Kit ELISA, MIF Kit ELISA, MRP8 Kit ELISA, NIF Kit ELISA, P8 Kit ELISA, Mrp8 Kit ELISA, S100A8 Kit ELISA, 60B8Ag Kit ELISA, AI323541 Kit ELISA, B8Ag Kit ELISA, CFAg Kit ELISA, Caga Kit ELISA, p8 Kit ELISA, MRP-8 Kit ELISA, S100 calcium binding protein A8 Kit ELISA, S100 calcium binding protein A8 (calgranulin A) Kit ELISA, S100A8 Kit ELISA, S100a8 Kit ELISA

ID gène: 20201

UniProt: P27005

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins