PKC epsilon Protein (AA 580-737) (His tag)

N° du produit ABIN1097337

Détail du produit

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Type de proteíne: Recombinant

Attributs du protein: AA 580-737

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette PKC epsilon protéine est marqué à la His tag.

Fonction: Recombinant Human Protein Kinase C Type ε/PKC ε/PKCE (C-6His)

Séquence: MQELEYGPSV DWWALGVLMY EMMAGQPPFE ADNEDDLFES ILHDDVLYPV WLSKEAVSIL KAFMTKNPHK RLGCVASQNG EDAIKQHPFF KEIDWVLLEQ KKIKPPFKPR IKTKRDVNNF DQDFTREEPV LTLVDEAIVK QINQEEFKGF SYFGEDLMPL EHHHHHH

Attributs du produit: Recombinant Human PKC epsilon is produced with our E. coli expression system. The target protein is expressed with sequence (Gln580-Pro737) of Human PRKCE.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: -80 °C

Stockage commentaire: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Autre désignation: PKC epsilon (PRKCE Produits)

Synonymes: PKCE Protein, nPKC-epsilon Protein, 5830406C15Rik Protein, PKC[e] Protein, PKCepsilon Protein, Pkce Protein, R75156 Protein, fj59b07 Protein, prkce Protein, wu:fj59b07 Protein, pkce Protein, pkc-epsilon Protein, PRKCH Protein, protein kinase C epsilon Protein, protein kinase C, epsilon Protein, protein kinase C, epsilon a Protein, PRKCE Protein, Prkce Protein, prkcea Protein, prkce Protein

Sujet: Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain, one C2 domain, one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters, a class of tumor promoters.ReferencesZhang KL, et al. Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas.PMID: 24012640http://www.ncbi.nlm.nih.gov/pubmed/24012640
Alternative Names: Protein Kinase C Epsilon Type, nPKC-Epsilon, PRKCE, PKCE

Poids moléculaire: 19.64 kDa

UniProt: Q02156

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling