p53 Protein (full length)

N° du produit ABIN2669408

Cette protéine Recombinant p53 est exprimée dans Baculovirus.

Aperçu rapide pour p53 Protein (full length) (ABIN2669408)

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus

Application: ELISA, Protein Interaction (PI)

Détail du produit

Attributs du protein: full length

Attributs du produit: Full-length recombinant p53 protein was expressed in a baculovirus system with a polyhistidine tag and purified by an affinity column in combination with FPLC chromatography. Active Motif also offers Recombinant p53 protein (Catalog No. 31465) that was expressed in E. coli.

Purification: Purified by an affinity column in combination with FPLC chromatography.

Information d'application

Indications d'application: Recombinant p53 is suitable for TransAM®, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40 - 0.625 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Stock: -20 °C/-80 °C

Stockage commentaire: Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Détail du antigène

Antigène: p53 (TP53) (Tumor Protein P53 (TP53))

Autre désignation: p53

Sujet: P53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis.

Pathways: Signalisation p53, Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, L'effet Warburg