LRP6 Protein (AA 20-1370) (His tag)

N° du produit ABIN3132152

Détail du produit

Antigène: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Type de proteíne: Recombinant

Attributs du protein: AA 20-1370

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette LRP6 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: APLLLYANRR DLRLVDATNG KENATIVVGG LEDAAAVDFV FGHGLIYWSD VSEEAIKRTE FNKSESVQNV VVSGLLSPDG LACDWLGEKL YWTDSETNRI EVSNLDGSLR KVLFWQELDQ PRAIALDPSS GFMYWTDWGE VPKIERAGMD GSSRFVIINT EIYWPNGLTL DYQERKLYWA DAKLNFIHKS NLDGTNRQAV VKGSLPHPFA LTLFEDTLYW TDWNTHSILA CNKYTGEGLR EIHSNIFSPM DIHAFSQQRQ PNATNPCGID NGGCSHLCLM SPVKPFYQCA CPTGVKLMEN GKTCKDGATE LLLLARRTDL RRISLDTPDF TDIVLQLEDI RHAIAIDYDP VEGYIYWTDD EVRAIRRSFI DGSGSQFVVT AQIAHPDGIA VDWVARNLYW TDTGTDRIEV TRLNGTMRKI LISEDLEEPR AIVLDPMVGY MYWTDWGEIP KIERAALDGS DRVVLVNTSL GWPNGLALDY DEGTIYWGDA KTDKIEVMNT DGTGRRVLVE DKIPHIFGFT LLGDYVYWTD WQRRSIERVH KRSAEREVII DQLPDLMGLK ATSVHRVIGS NPCAEDNGGC SHLCLYRPQG LRCACPIGFE LIGDMKTCIV PEAFLLFSRR ADIRRISLET NNNNVAIPLT GVKEASALDF DVTDNRIYWT DISLKTISRA FMNGSALEHV VEFGLDYPEG MAVDWLGKNL YWADTGTNRI EVSKLDGQHR QVLVWKDLDS PRALALDPAE GFMYWTEWGG KPKIDRAAMD GSERTTLVPN VGRANGLTID YAKRRLYWTD LDTNLIESSD MLGLNREVIA DDLPHPFGLT QYQDYIYWTD WSRRSIERAN KTSGQNRTII QGHLDYVMDI LVFHSSRQAG WNECASSNGH CSHLCLAVPV GGFVCGCPAH YSLNADNRTC SAPSTFLLFS QKSAINRMVI DEQQSPDIIL PIHSLRNVRA IDYDPLDKQL YWIDSRQNSI RKAHEDGGQG FNVVANSVAN QNLEIQPYDL SIDIYSRYIY WTCEATNVID VTRLDGRSVG VVLKGEQDRP RAIVVNPEKG YMYFTNLQER SPKIERAALD GTEREVLFFS GLSKPIALAL DSKLGKLFWA DSDLRRIESS DLSGANRIVL EDSNILQPVG LTVFENWLYW IDKQQQMIEK IDMTGREGRT KVQARIAQLS DIHAVKELNL QEYRQHPCAQ DNGGCSHICL VKGDGTTRCS CPMHLVLLQD ELSCGEPPTC SPQQFTCFTG DIDCIPVAWR CDGFTECEDH SDELNCPVCS ESQFQCASGQ CIDGALRCNG DANCQDKSDE KNCEVLCLID QFRCANGQCV GKHKKCDHSV DCSDRSDELD CYPTEEPAPQ A
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Lrp6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Autre désignation: Lrp6 (LRP6 Produits)

Synonymes: lrp6-A Protein, Xlrp6 Protein, adcad2 Protein, lrp6 Protein, LRP6 Protein, ADCAD2 Protein, C030016K15Rik Protein, Cd Protein, Gw Protein, LDL receptor related protein 6 Protein, LDL receptor related protein 6 L homeolog Protein, LDL receptor related protein 6 S homeolog Protein, low density lipoprotein receptor-related protein 6 Protein, low-density lipoprotein receptor-related protein 6 Protein, LRP6 Protein, lrp6.L Protein, lrp6.S Protein, lrp6 Protein, Lrp6 Protein, LOC590395 Protein

Sujet: Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation (By similarity). {ECO:0000250, ECO:0000269|PubMed:15142971}.

Poids moléculaire: 153.0 kDa Including tag.

UniProt: O88572

Pathways: Signalisation WNT, Tube Formation