TAB2 Protein (AA 1-693) (Strep Tag)

N° du produit ABIN3136942

Détail du produit

Antigène: TAB2 (TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 2 (TAB2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-693

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TAB2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAQGSHQIDF QVLHDLRQKF PEVPEVVVSR CMLQNNNNLD ACCAVLSQES TRYLYGEGDL NFSDESGISG LRNHMTSLNL DLQSQNVYHH GREGSRVNGS RTLTHSVSDG QLHGGQSNNE LFQQEPQTAP AQVPQGFNVF GMPSTSGASN STPHLGFHLG SKGTSNLSQQ TPRFNPIMVT LAPNIQTGRS TPTSLHIHGV PPPVLNSPQG NSIYIRPYIT TPSGTARQTQ QHSGWVSQFN PMNPQQAYQP SQPGPWTTYP ASNPLPHTST QQPNQQGHQT SHVYMPISSP TTPQPPTIHS SGSSQSSAHS QYNIQNISTG PRKNQIEIKL EPPQRNSSSK LRSSGPRTAS TSSLVNSQTL NRNQPTVYIA ASPPNTDEMI SRSQPKVYIS ANATAGDEQG MRNQPTLFIS TNSGPSAASR NMSGQVSMGP AFIHHHPPKS RVLGGNSATS PRVVVTQPNT KYTFKITVSP NKPPAVSPGV VSPTFELTNL LNHPDHYVET ENIQHLTDPA LAHVDRISEA RKLSMGSDDA AYTQALLVHQ KARMERLQRE LEMQKKKLDK LKSEVNEMEN NLTRRRLKRS NSISQIPSLE EMQQLRSCNR QLQIDIDCLT KEIDLFQARG PHFNPSAIHN FYDNIGFVGP VPPKPKDQRS TIKAPKTQDA EDEEGAQWNC TACTFLNHPA LIRCEQCEMP RHF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TAB2 (TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 2 (TAB2))

Autre désignation: Tab2 (TAB2 Produits)

Synonymes: CHTD2 Protein, MAP3K7IP2 Protein, 1110030N06Rik Protein, A530078N03Rik Protein, Map3k7ip2 Protein, mKIAA0733 Protein, map3k7ip2 Protein, zgc:77446 Protein, TGF-beta activated kinase 1/MAP3K7 binding protein 2 Protein, TAB2 Protein, Tab2 Protein, tab2 Protein

Sujet: TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 (Mitogen-activated protein kinase kinase kinase 7-interacting protein 2) (TAK1-binding protein 2) (TAB-2) (TGF-beta-activated kinase 1-binding protein 2),FUNCTION: Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF) (By similarity). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains (By similarity). The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2: this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (PubMed:19927120). Regulates the IL1-mediated translocation of NCOR1 out of the nucleus (PubMed:12150997). Involved in heart development (By similarity). {ECO:0000250|UniProtKB:Q9NYJ8, ECO:0000269|PubMed:12150997, ECO:0000269|PubMed:19927120}.

Poids moléculaire: 76.4 kDa

UniProt: Q99K90

Pathways: TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway