TGFA Protein (Transcript Variant 1)

N° du produit ABIN5505784

Détail du produit

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: Transcript Variant 1

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human TGF-alpha (TGFA) (transcript variant 1) protein expressed in E.coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Pureté: > 98 % , as determined by Coomassie stained SDS-PAGE.

niveau d'endotoxine: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.1 - 0.4 ng/ml, corresponding to a specific activity of 0.25 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of BALB/c-3T3 mouse embryonic fibroblast cells proliferation.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Concentration: > 50 μg/mL

Buffer: 1 x PBS

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: TGFA (Transforming Growth Factor, alpha (TGFA))

Autre désignation: TGF-alpha (TGFA Produits)

Synonymes: TFGA Protein, LOC100009482 Protein, TGF alpha Protein, TGFA Protein, wa-1 Protein, wa1 Protein, RATTGFAA Protein, TGFAA Protein, NV15606 Protein, si:ch211-10e8.7 Protein, transforming growth factor alpha Protein, transforming growth factor alpha L homeolog Protein, transforming growth factor-alpha Protein, transforming growth factor, alpha Protein, TGFA Protein, TGF-A Protein, Tgfa Protein, tgfa.L Protein, LOC100121300 Protein, LOC100009150 Protein, tgfa Protein

Sujet: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. [UniProtKB/Swiss-Prot Function]

Poids moléculaire: 5.5 kDa

NCBI Accession: NP_003227

Pathways: Signalisation NF-kappaB, Signalisation RTK, EGFR Signaling Pathway