KIT Ligand Protein (KITLG) (AA 25-191) (His tag)

N° du produit ABIN6239749

Détail du produit

Antigène: KIT Ligand (KITLG)

Activité biologique: Active

Attributs du protein: AA 25-191

Origine: Porc

Source: HEK-293 Cells

Purification/Conjugué: Cette KIT Ligand protéine est marqué à la His tag.

Application: Cell Culture (CC), Activity Assay (AcA)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x104cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37oC. The growth of cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. Cell proliferation of TF-1 cells after incubation with SCF for 72h observed by inverted microscope was shown in Figure 1.

Information d'application

Indications d'application: Isoelectric Point: 5.3

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: Stem Cell Factor (SCF) (KITLG Produits)

Synonymes: Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein

Sujet: Alternative Names: KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2

Poids moléculaire: 20kDa

UniProt: P20826

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway