CD25 Protein (AA 22-236) (His tag)
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- Antigène Voir toutes CD25 (IL2RA) Protéines
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 22-236
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Origine
- Souris
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette CD25 protéine est marqué à la His tag.
- Fonction
- Mouse IL-2 R alpha/CD25 Protein
- Séquence
- Glu22-Lys236
- Attributs du produit
- Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Mouse IL-2 R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA. The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- Autre désignation
- IL-2 R alpha (IL2RA Produits)
- Synonymes
- CD25 Protein, IDDM10 Protein, IL2R Protein, TCGFR Protein, IL2RAC Protein, IL-2RA Protein, Il2r Protein, Ly-43 Protein, interleukin 2 receptor subunit alpha Protein, interleukin-2 receptor subunit alpha-like Protein, interleukin 2 receptor, alpha chain Protein, IL2RA Protein, Il2ra Protein, LOC100222957 Protein
- Sujet
- IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL-2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
- Poids moléculaire
- 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P01590
- Pathways
- Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation
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