Cette EGFRviii protéine est marqué à la His-Avi Tag,Biotin.
Fonction
Biotinylated Human EGFRVIII Protein
Séquence
Leu25-Ser378
Attributs du produit
Recombinant Biotinylated Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.
Pureté
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human EGFRVIII, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Poids moléculaire
41.6 kDa. Due to glycosylation, the protein migrates to 60-78 kDa based on Tris-Bis PAGE result.