Cette Syndecan 1 protéine est marqué à la His tag.
Fonction
Human Syndecan-1 Protein
Séquence
Gln23-Gly254
Attributs du produit
Recombinant Human Syndecan-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Gly254.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Syndecan-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA. See testing image for detail.
SDC1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver.
Poids moléculaire
25 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result.