Recombinant Cynomolgus c-MPL/Thrombopoietin R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Thr489.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus c-MPL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-C-MPL Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
MPL
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
MPL
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
SDS, WB, Imm, PC
Restrictions
For Research Use only
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10 % Glycerol ( pH 7.5).
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-80 °C
Stockage commentaire
Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The c-mpl gene encodes a member of the hematopoietic cytokine receptor superfamily. This gene was discovered through the study of a murine retrovirus which induces an acute myeloproliferative syndrome in mice.MPLV (for myeloproliferative leukemia virus) has transduced a truncated and constitutively activated form of the c-mpl receptor chain. High levels of c-mpl expression were detected in 45 % of acute myeloid leukemia (AML). No correlation was found between c-mpl expression and the French-American-British classification subtype of AML.
Poids moléculaire
53.48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.