Purified recombinant Human SCF protein Expression System: E.coli Bioactivity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/mL, corresponding to a Specific Activity of 500,000 IU/mg.
KITLG
Origine: Rat
Hôte: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Indications d'application
Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute the lyophilized Stem Cell Factor in sterile water not less than 100 µg/mL.
Buffer
Supplied in lyophilized form containing 10 mM Acetic acid,
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
-20 °C
Stockage commentaire
Stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution SCF protein should be stored at 4 °C for short term use. For long term storage it is recommended to add a carrier protein (0.1 % HSA or BSA) and store below -18 °C.
Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on Mouse cells, while Mouse and rat SCF are fully active on human cells. Alternative Names: Steel Factor protein, MGF protein, c-Kit Ligand protein, Mast Cell Growth Factor protein, Stem Cell Factor protein