HMGB1 Protein (His tag)

N° du produit ABIN987946

Détail du produit

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette HMGB1 protéine est marqué à la His tag.

Séquence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HH

Pureté: > 95 % by SDS-PAGE and HPLC analyses.

niveau d'endotoxine: Level Less than 1EU/µg of rHuHMGB1 as determined by LAL method

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stock: 4 °C

Référence

Dikeakos, Thomas, Kwon, Elferich, Shinde, Thomas: "An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS-2 on endosomes to down-regulate MHC-I." dans: Molecular biology of the cell, Vol. 23, Issue 11, pp. 2184-97, (2012) (PubMed).

Contreras, Friday, Morrison, Hao, Keiper: "Cap-independent translation promotes C. elegans germ cell apoptosis through Apaf-1/CED-4 in a caspase-dependent mechanism." dans: PLoS ONE, Vol. 6, Issue 9, pp. e24444, (2011) (PubMed).

Détail du antigène

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-Mobility Group Box 1 (HMGB1) (HMGB1 Produits)

Synonymes: HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein

Sujet: Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. Synonym: High-Mobility Group Box 1 (His6-tagged) ( HMGB1), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Poids moléculaire: Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 223 amino acids.

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome