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IKBKB anticorps (AA 1-666)

Name: IKBKB anticorps (AA 1-666)
SKU: ABIN967355
Availability: InStock

Il existe 10+ publications pour ce produit. L’anticorps anti-IKBKB Monoclonal Souris est utilisé pour la détection de IKBKB dans des échantillons de Humain. Il a été validé pour WB et IP.

Western blot analysis of Ikkbeta. (IKBKB anticorps (AA 1-666))

1 image

(10 références)

N° du produit ABIN967355

297,34 €

Plus frais de livraison 40,00 € et TVA

50 μg

Destination: France

Envoi sous 12 à 15 jours ouvrables

Aperçu rapide pour IKBKB anticorps (AA 1-666) (ABIN967355)

Antigène

Voir toutes IKBKB Anticorps

IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivité

Humain

Hôte

Souris

Clonalité

Monoclonal

Conjugué

Cet anticorp IKBKB est non-conjugé

Application

Western Blotting (WB), Immunoprecipitation (IP)

Clone

F18-1875

Discover our top product IKBKB Anticorps primaire

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AA 1-666

Attributs du produit

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène

Human IKKbeta recombinant protein aa. 1-666

Isotype

IgG1
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (AA 630-730) antibody
IKBKB Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (C-Term) antibody
IKBKB Reactivité: Humain WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (pTyr199) antibody
IKBKB Reactivité: Humain, Souris, Rat, Singe WB, ELISA, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody
IKBKB Reactivité: Humain WB, IF, IHC (p) Hôte: Lapin Polyclonal RB2161 unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (AA 3-120) antibody
IKBKB Reactivité: Humain WB, ELISA, PLA Hôte: Souris Monoclonal 1A3 unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody
IKBKB Reactivité: Humain WB, IHC, IF Hôte: Lapin Monoclonal unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody
IKBKB Reactivité: Humain WB, ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (AA 3-120) antibody
IKBKB Reactivité: Humain WB, ELISA, IF Hôte: Souris Monoclonal 3C12 unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) (pTyr188) antibody
IKBKB Reactivité: Humain, Souris, Rat, Singe WB, ELISA, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB) antibody
IKBKB Reactivité: Humain WB, ELISA, IHC, IF Hôte: Lapin Monoclonal 3E1 unconjugated Recombinant Antibody

Indications d'application

Applications include western blot analysis (0.06 - 0.25 µg/ml). Other applications not routinely tested include immunoprecipitation (4 µg/sample). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (store lysate at -20°C). HeLa control lysate (ABIN968535) is sold separately as a ready-to-use western blot control.

Commentaires

Related Products: ABIN967389, ABIN968535

Restrictions

For Research Use only
Format

Liquid

Concentration

0.25 mg/mL

Buffer

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C

Stockage commentaire

Store undiluted at -20°C.
Israël: "The IKK complex: an integrator of all signals that activate NF-kappaB?" dans: Trends in cell biology, Vol. 10, Issue 4, pp. 129-33, (2000) (PubMed).

Chu, Ostertag, Li, Chang, Chen, Hu, Williams, Perrault, Karin: "JNK2 and IKKbeta are required for activating the innate response to viral infection." dans: Immunity, Vol. 11, Issue 6, pp. 721-31, (2000) (PubMed).

Li, Van Antwerp, Mercurio, Lee, Verma: "Severe liver degeneration in mice lacking the IkappaB kinase 2 gene." dans: Science (New York, N.Y.), Vol. 284, Issue 5412, pp. 321-5, (1999) (PubMed).

Li, Chu, Hu, Delhase, Deerinck, Ellisman, Johnson, Karin: "The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis." dans: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1839-45, (1999) (PubMed).

Tanaka, Fuentes, Yamaguchi, Durnin, Dalrymple, Hardy, Goeddel: "Embryonic lethality, liver degeneration, and impaired NF-kappa B activation in IKK-beta-deficient mice." dans: Immunity, Vol. 10, Issue 4, pp. 421-9, (1999) (PubMed).

Ling, Cao, Goeddel: "NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3792-7, (1998) (PubMed).

Nakano, Shindo, Sakon, Nishinaka, Mihara, Yagita, Okumura: "Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3537-42, (1998) (PubMed).

Rothwarf, Zandi, Natoli, Karin: "IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex." dans: Nature, Vol. 395, Issue 6699, pp. 297-300, (1998) (PubMed).

Lee, Hagler, Chen, Maniatis: "Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway." dans: Cell, Vol. 88, Issue 2, pp. 213-22, (1997) (PubMed).

Malinin, Boldin, Kovalenko, Wallach: "MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1." dans: Nature, Vol. 385, Issue 6616, pp. 540-4, (1997) (PubMed).
Antigène

IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Autre désignation

IKK beta

Sujet

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. The nuclear transcription factor kappa-B, NF-kappaB, is controlled by interaction with an inhibitory subunit, I-kappaB, which restricts NF-kappaB to the cytoplasm. Following stimulation by various cytokines or other stimuli, I-kappaB becomes degraded and NF-kappaB is released to the nucleus. The release of I-kappaB from NF-kappaB is thought to be a critical point in the activation of NF-kappaB signal pathways. A group of proteins form an NF-kappaB regulatory complex, or signalsome. Two members of this complex are a pair of closely related serine/threonine kinases, IKKalpha and IKKbeta (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-kappaB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKalpha, IKKbeta, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKgamma. IKKalpha and IKKbeta become activated following phosphorylation by upstream kinases, including NF-kappaB-inducing kinase (NIK) and MEKK1. IKKgamma interacts preferentially with IKKbeta and is required for the activation of the IKK complex. Experiments done on mice lacking IKKbeta demonstrate that these mice died at mid-gestation from uncontrolled liver apoptosis. Furthermore, when cells from IKKbeta-deficient m ice were treated with tumor necrosis factor alpha or interleukin 1, neither IKK nor NF-kappaB were activated. Thus IKKbeta, but not IKKalpha, plays a pivotal role in the induction of NF-kappaB and activation of IKK. IKKbeta migrates at ~87 kDa in SDS/PAGE. The antibody recognizes human IKKbeta. A recombinant protein corresponding to amino acids 1-666 of human IKKbeta was used as the immunogen.

Pathways

Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins