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AKT1 anticorps (C-Term)

Cet anticorps Lapin Polyclonal détecte spécifiquement AKT1 dans WB, IF, IHC (p) et FACS. Il présente une réactivité avec des échantillons de Humain et Souris.

AKT1 Antibody (C-term ) (ABIN655915 and ABIN2845314) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). (AKT1 anticorps (C-Term))

FACS

Confocal immunofluorescent analysis of AKT1 Antibody (C-term ) (ABIN655915 and ABIN2845314) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (AKT1 anticorps (C-Term))

AKT1 Antibody (p) (ABIN655915 and ABIN2845314) western blot analysis in MCF-7 cell line lysates (35 μg/lane). (AKT1 anticorps (C-Term))

AKT1 Antibody (C-term ) (ABIN655915 and ABIN2845314) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. (AKT1 anticorps (C-Term))

IHC (p)

5 images

N° du produit ABIN655915

434,77 €

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Destination: France

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Aperçu rapide pour AKT1 anticorps (C-Term) (ABIN655915)

Antigène

Voir toutes AKT1 Anticorps

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité

Humain, Souris

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp AKT1 est non-conjugé

Application

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone

RB29255

Discover our top product AKT1 Anticorps primaire

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AA 428-457, C-Term

Homologie

B, Rat

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène

This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-457 amino acids from the C-terminal region of human AKT1.

Isotype

Ig Fraction
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 Reactivité: Humain WB, IHC, IF, IP Hôte: Lapin Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 122-443) antibody
AKT1 Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 Reactivité: Humain WB, ELISA, IF, IHC (p), IP Hôte: Souris Monoclonal 2E11 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
AKT1 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibody
AKT1 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody
AKT1 Reactivité: Humain, Souris, Rat, Chien, Singe WB, IHC, IF, IHC (p), FACS Hôte: Souris Monoclonal 3E3 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibody
AKT1 Reactivité: Humain WB, IHC, ELISA, IF, IP Hôte: Lapin Polyclonal unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 Reactivité: Humain, Souris, Rat, Singe WB, ELISA, FACS, ICC Hôte: Souris Monoclonal 1F7E10 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-150) antibody
AKT1 Reactivité: Humain, Rat, Singe WB, IHC, ELISA, FACS Hôte: Souris Monoclonal 1F7G2 unconjugated

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 381-480) antibody
AKT1 Reactivité: Humain ELISA, IP, PLA, RNAi Hôte: Souris Monoclonal 4C3 unconjugated

Indications d'application

IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Antigène

AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation

AKT1

Sujet

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Poids moléculaire

55686

ID gène

207

NCBI Accession

NP_001014431, NP_001014432, NP_005154

UniProt

P31749

Pathways

Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg