IKK alpha anticorps (pSer176, Ser180)

N° du produit ABIN220384

Cet anticorps Lapin Polyclonal détecte spécifiquement IKK alpha dans WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour IKK alpha anticorps (pSer176, Ser180) (ABIN220384)

Antigène: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IKK alpha est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-IKK alpha anticorps

Épitope: pSer176, Ser180

Specificité: Recognizes human IKKa. Species sequence homology: mouse-100 % . Sequence homology: IKKb-93 %.

Purification: Affinity purified

Immunogène: Synthetic phosphopeptide derived from the region of human IKKa that contains serine 176 and serine 180. The sequence is conserved in mouse.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: WB (0.5 - 1.5 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot: 0.5-1.5 μg/mL. at 0.75 μg/mL, the dilution provides 100 mL working solution, which 10 ml/blot allows 10 blots to be performed. When examining phosphorylation of endogenous IKKa protein, we recommend that IKKa first be immunoprecipitated. Positive control: Immunoprecipitates prepared from lysates of Jurkat cells, treated with TNF-a.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS (without Mg2+, Ca2+), 1 mg/mL BSA (IgG, protease free),  pH 7.3, 0.05 % sodium azide, before tha addition of glycerol to 40 %.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur IKK alpha

Antigène: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Autre désignation: CHUK / IKKA / IKK Alpha

Sujet: Name/Gene ID: CHUK
Subfamily: IKAPPAB
Family: Protein Kinase

Synonyms: CHUK, I-kappa-B kinase alpha, I-kappa-B kinase-alpha, Ikb kinase alpha, IKK-A, IKBKA, IKK1, NFKBIKA, I-kappa-B kinase 1, IkappaB kinase, IKK Alpha, IKK-alpha, IKKA, Transcription factor 16, IkappaBalpha Kinase, IkB kinase alpha subunit, IKK-a kinase, Ikkalpha, TCF-16, TCF16

ID gène: 1147

UniProt: O15111

Pathways: Signalisation PI3K-Akt, Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins