SLC2A4
Reactivité: Humain, Souris, Rat
WB, ELISA, IHC (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1 : 1000 (AP staining) IP: not tested yet ICC: not tested yet IHC-P: 1 : 200
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Conseil sur la manipulation
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Stock
-20 °C
Stockage commentaire
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Roccisana, Sadler, Bryant, Gould: "Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45." dans: Molecular biology of the cell, Vol. 24, Issue 15, pp. 2389-97, (2013) (PubMed).
Boyle, Logan, Jones, Small, Sattar, Connell, Cleland, Salt: "AMP-activated protein kinase is activated in adipose tissue of individuals with type 2 diabetes treated with metformin: a randomised glycaemia-controlled crossover study." dans: Diabetologia, Vol. 54, Issue 7, pp. 1799-809, (2011) (PubMed).
Antigène
GLUT4 (SLC2A4)
(Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))