STAT3 anticorps (AA 688-722)
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- Antigène Voir toutes STAT3 Anticorps
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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Épitope
- AA 688-722
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache), Porc, Singe, Cheval, Hamster
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp STAT3 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Gel Shift (GS)
- Specificité
- Recognizes STAT3 (92kD). A second unknown band at 50kD may be observed with longer exposures or at high concentrations of the antibody. Species cross-reactivity: Human, rat and mouse.
- Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%) Sheep (97%) Chicken (84%).
- Purification
- Protein A purified
- Immunogène
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Bacterially expressed GST fusion protein corresponding to human STAT3 (aa688-722, RPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTI). The immunizing sequence is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Horse, Pig, Opossum (100%), Sheep (97%), Chicken (84%).
Type of Immunogen: Fusion protein - Isotype
- IgG
- Top Product
- Discover our top product STAT3 Anticorps primaire
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- Indications d'application
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Approved: GS, ICC (10 μg/mL), IP, WB (2 - 4 μg/mL)
Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Western Blot: 2-4 μg/mL detects STAT3 in RIPA lysates from EGF stimulated human A431 cells. EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT3 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 10 μg/mL shows positive immunostaining for STAT 3 in A431 cells fixed with 95 % ethanol, 5 % acetic acid. Immunoprecipitation: 4 μg immunoprecipitates STAT 3 from 500 μg of EGF-stimulated A431 RIPA lysate. Gel Shift Assay: This antibody supershifts. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 40 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Antigène
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
- Autre désignation
- STAT3 (STAT3 Produits)
- Synonymes
- anticorps 1110034C02Rik, anticorps AW109958, anticorps Aprf, anticorps APRF, anticorps HIES, anticorps Xstat3, anticorps aprf, anticorps hies, anticorps stat3, anticorps wu:fc15d02, anticorps wu:fl59g06, anticorps z-Stat3, anticorps signal transducer and activator of transcription 3, anticorps signal transduction and activation of transcription 3, anticorps signal transducer and activator of transcription 3, gene 1 L homeolog, anticorps signal transducer and activator of transcription 3 (acute-phase response factor), anticorps STAT3, anticorps stat3, anticorps Stat3, anticorps stat3.1.L
- Sujet
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Name/Gene ID: STAT3
Synonyms: STAT3, Acute-phase response factor, APRF, DNA-binding protein APRF, HIES - ID gène
- 6774
- UniProt
- P40763
- Pathways
- Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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