AKT1 anticorps (Internal Region)

N° du produit ABIN233853

N° du produit (Fournisseur): 600-601-401

L’anticorps anti-AKT1 Polyclonal Mouton est utilisé pour la détection de AKT1 dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA, FACS et FM.

Aperçu rapide pour AKT1 anticorps (Internal Region) (ABIN233853)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Détail du produit anti-AKT1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-601-401

Fournisseur: Rockland

Fonction: AKT Antibody

Réactivité croisée (Details): This antibody is directed against AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT.

Attributs du produit: Synonyms: sheep anti-AKT Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein.  A residue of cysteine was added to facilitate coupling.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Flow Cytometry Dilution: User Optimized

Immunohistochemistry Dilution: User Optimized

Application Note: This Anti-AKT affinity purified antibody is tested for ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user for IF and IHC.  Expect a band approximately 55-60 kDa in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract. Cell Signaling, Neuroscience, Signal Transduction research.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.9 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Anti-AKT at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1

Sujet: Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

ID gène: 207, 62241011

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg