AKT1 anticorps

N° du produit ABIN265302

Détail du produit anti-AKT1 anticorps

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody detects endogenous levels of AKT1 / PKB protein (region surrounding Asp302).

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1 / PKB (AKT1 Produits)

Synonymes: anticorps AKT, anticorps CWS6, anticorps PKB, anticorps PKB-ALPHA, anticorps PRKBA, anticorps RAC, anticorps RAC-ALPHA, anticorps Akt, anticorps Rac, anticorps PKB/Akt, anticorps PKBalpha, anticorps AKT/PKB, anticorps AKT1, anticorps Akt/PKB, anticorps Akt1, anticorps CG4006, anticorps D-Akt, anticorps DAKT1, anticorps DAKT1/PKB, anticorps DAkt, anticorps DAkt1, anticorps DPKB, anticorps DRAC-PK, anticorps DRAC-PK66, anticorps DRAC-PK85, anticorps Dakt, anticorps Dakt1, anticorps Dmel\\CG4006, anticorps Dpkb, anticorps PKB/AKT, anticorps PKB/dAKT, anticorps RacPK, anticorps akt, anticorps akt1, anticorps dAKT, anticorps dAKT/dPKB, anticorps dAKT1, anticorps dAkt, anticorps dAkt/PKB, anticorps dAkt1, anticorps dPKB, anticorps dakt, anticorps dakt1, anticorps l(3)04226, anticorps l(3)89Bq, anticorps p-Akt, anticorps pAkt, anticorps ACT-5, anticorps akt-1, anticorps pkb, anticorps v-akt, anticorps v-akt1, anticorps xAct, anticorps ATAKT1, anticorps F18A8.2, anticorps F18A8_2, anticorps K+ transporter 1, anticorps POTASSIUM TRANSPORTER, anticorps AKT serine/threonine kinase 1, anticorps thymoma viral proto-oncogene 1, anticorps CG4006 gene product from transcript CG4006-RE, anticorps actin beta, anticorps v-akt murine thymoma viral oncogene homolog 1 S homeolog, anticorps v-akt murine thymoma viral oncogene homolog 1, anticorps K+ transporter 1, anticorps Serine/threonine-protein kinase akt-1, anticorps AKT1, anticorps Akt1, anticorps ACTB, anticorps akt1.S, anticorps akt1, anticorps KT1, anticorps akt-1

Sujet: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase

Poids moléculaire: approx. 60 kDa

ID gène: 207

NCBI Accession: NP_001014431

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg