LAT anticorps

N° du produit ABIN272032

Détail du produit anti-LAT anticorps

Antigène: LAT (Linker For Activation of T Cells (LAT))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LAT est non-conjugé

Application: Western Blotting (WB)

Specificité: This antibody detects endogenous levels of LAT protein. (region surrounding Leu185)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur LAT

Antigène: LAT (Linker For Activation of T Cells (LAT))

Autre désignation: LAT (LAT Produits)

Synonymes: anticorps LAT, anticorps lat, anticorps LAT1, anticorps pp36, anticorps linker for activation of T-cells, anticorps linker for activation of T cells, anticorps LAT, anticorps lat, anticorps Lat

Sujet: LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36

Poids moléculaire: approx. 28 kDa

ID gène: 27040

NCBI Accession: NP_001014987

UniProt: O43561

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway