SMAD2
Reactivité: Humain, Souris, Rat
WB, IHC (p), ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C,-20 °C
Liu, Pouponnot, Massagué: "Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes." dans: Genes & development, Vol. 11, Issue 23, pp. 3157-67, (1998) (PubMed).
Takenoshita, Mogi, Nagashima, Yang, Yagi, Hanyu, Nagamachi, Miyazono, Hagiwara: "Characterization of the MADH2/Smad2 gene, a human Mad homolog responsible for the transforming growth factor-beta and activin signal transduction pathway." dans: Genomics, Vol. 48, Issue 1, pp. 1-11, (1998) (PubMed).
Zhang, Feng, We, Derynck: "Receptor-associated Mad homologues synergize as effectors of the TGF-beta response." dans: Nature, Vol. 383, Issue 6596, pp. 168-72, (1996) (PubMed).
Antigène
SMAD2
(SMAD, Mothers Against DPP Homolog 2 (SMAD2))
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.