SMAD2 anticorps (N-Term)

N° du produit ABIN3044112

Détail du produit anti-SMAD2 anticorps

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Épitope: AA 94-112, N-Term

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: DQWDTTGLYS FSEQTRSLD

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: SMAD family member 2
Protein Name: Mothers against decapentaplegic homolog 2

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2(94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SMAD2 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Références pour anticorps anti-SMAD2 (ABIN3044112)

Xu, Xu, Saud, Lu, Liu, Fang, Zhang, Hu, Li: "Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2601830, (2016) (PubMed).

Tao, Hu, Li, Liu, Wu, Li, Fu, Wei, Luo: "Tranilast prevents the progression of chronic cyclosporine nephrotoxicity through regulation of transforming growth factor ?/Smad pathways." dans: Transplantation proceedings, Vol. 43, Issue 5, pp. 1985-8, (2011) (PubMed).

Détails sur SMAD2

Antigène: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Autre désignation: SMAD2 (SMAD2 Produits)

Synonymes: anticorps JV18, anticorps JV18-1, anticorps MADH2, anticorps MADR2, anticorps hMAD-2, anticorps hSMAD2, anticorps jv18, anticorps Xmad2, anticorps madh2, anticorps madr2, anticorps XSmad2, anticorps hmad-2, anticorps hsmad2, anticorps jv18-1, anticorps smad-2, anticorps fj43c06, anticorps wu:fj43c06, anticorps 7120426M23Rik, anticorps Madh2, anticorps Madr2, anticorps Smad-2, anticorps mMad2, anticorps MAD homolog 2, anticorps Mothers against DPP homolog 2, anticorps SMAD 2, anticorps Smad2, anticorps SMAD family member 2, anticorps SMAD family member 2-Z, anticorps SMAD family member 2 S homeolog, anticorps SMAD2, anticorps SMAD2Z, anticorps smad2, anticorps smad2.S, anticorps Smad2

Sujet: Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.

Synonyms: hMAD 2 antibody|hMAD-2 antibody|hSMAD2 antibody|JV18 1 antibody|JV18 antibody|JV18-1 antibody|JV181 antibody|MAD antibody|MAD homolog 2 antibody|MAD Related Protein 2 antibody|Mad-related protein 2 antibody|MADH2 antibody|MADR2 antibody|MGC22139 antibody|MGC34440 antibody|Mothers Against Decapentaplegic Homolog 2 antibody|mothers against DPP homolog 2 antibody|OTTHUMP00000163489 antibody|Sma and Mad related protein 2 antibody|SMAD 2 antibody|SMAD antibody|SMAD family member 2 antibody|SMAD, mothers against DPP homolog 2 antibody|SMAD2 antibody|SMAD2_HUMAN antibody

UniProt: Q15796

Pathways: Cycle Cellulaire, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus