XRCC1 anticorps (N-Term)

N° du produit ABIN3044234

Détail du produit anti-XRCC1 anticorps

Antigène: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Épitope: AA 15-34, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XRCC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Fonction: Rabbit IgG polyclonal antibody for DNA repair protein XRCC1(XRCC1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Séquence: QDSTHCAENL LKADTYRKWR

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for DNA repair protein XRCC1(XRCC1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Protein Name: DNA repair protein XRCC1

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1(15-34aa QDSTHCAENLLKADTYRKWR), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur XRCC1

Antigène: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Autre désignation: XRCC1 (XRCC1 Produits)

Synonymes: anticorps xrcc1, anticorps MGC80909, anticorps XRCC1, anticorps rcc, anticorps RCC, anticorps Xrcc-1, anticorps X-ray repair complementing defective repair in Chinese hamster cells 1, anticorps X-ray repair cross complementing 1, anticorps xrcc1, anticorps XRCC1, anticorps Xrcc1

Sujet: XRCC1(X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Synonyms: DNA repair protein XRCC1 antibody|RCC antibody|X ray repair complementing defective repair in chinese hamster antibody|X ray repair complementing defective repair in chinese hamster cells 1 antibody|X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody|X ray repair cross complementing protein 1 antibody|X ray repair, complementing defective, repair in Chinese hamster antibody|X-ray repair cross-complementing protein 1 antibody|XRCC 1 antibody|Xrcc1 antibody|XRCC1_HUMAN antibody

UniProt: P18887

Pathways: Réparation de l'ADN