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EGFR anticorps (Tyr1173)

Name: EGFR anticorps (Tyr1173)
Brand: antibodies-online
SKU: ABIN343717
Price: 414.05 EUR
Availability: InStock

EGFR Reactivité: Humain WB, FACS, IP Hôte: Souris Monoclonal EM-13 unconjugated

Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 inEGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions). (EGFR anticorps (Tyr1173))

Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified antibody (concentration in sample 3 μg/mL, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining). (EGFR anticorps (Tyr1173))

FACS

Immunoprecipitation ofEGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody (C)and anti-EGFR monoclonal mAb108 (D). (EGFR anticorps (Tyr1173))

Immunoprecipitation ofEGFR from EGF-treated A431 cells byphosphospecific monoclonal antibodies EM-12 (A), (B), a commercial anti-EGFR polyclonal antibody(C) and anti-EGFR monoclonal mAb108 (D). (EGFR anticorps (Tyr1173))

4 images

N° du produit ABIN343717

Fiche de données

Antigène Voir toutes EGFR Anticorps

EGFR (Epidermal Growth Factor Receptor (EGFR))
Épitope
41

39

39

38

36

28

28

27

22

21

21

19

18

17

16

16

15

15

15

14

14

13

13

11

9

8

7

7

7

6

5

5

5

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2
Tyr1173
Reactivité
818

330

322

26

26

24

21

15

9

3

3

2

1

1

1

1

1

1

1
Humain
Hôte
636

185

14

11

5

1
Souris
Anticorps secondaires appropriés
Clonalité
584

263

2
Monoclonal
Conjugué
432

63

32

31

31

30

29

27

27

25

24

16

10

10

10

10

10

5

4

4

4

4

4

2

1

1

1

1

1

1

1

1
Cet anticorp EGFR est non-conjugé
Application
567

264

150

149

140

102

101

96

81

55

37

34

34

19

10

8

6

4

4

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificité

The mouse monoclonal antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on intracellular tyrosine 1173.

Réactivité croisée (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Pureté

> 95 % (by SDS-PAGE)

Immunogène

Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.

Clone

EM-13

Isotype

IgG1

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Indications d'application

Western blotting: Recommended dilution: 1 μg/mL, positive control: EGF-stimulated A431, negative control: non-stimulated A431.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Do not freeze.

Stock

4 °C

Stockage commentaire

Store at 2-8°C. Do not freeze.
Antigène

EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation

EGFR (EGFR Produits)

Synonymes

anticorps C-erb, anticorps CG10079, anticorps D-EGFR, anticorps D-Egf, anticorps DEGFR, anticorps DER, anticorps DER flb, anticorps DER/EGFR, anticorps DER/faint little ball, anticorps DER/top, anticorps DER/torpedo, anticorps DER1, anticorps DEgfr, anticorps Degfr, anticorps Der, anticorps DmHD-33, anticorps Dmel\\CG10079, anticorps EFG-R, anticorps EGF-R, anticorps EGFR, anticorps EGFr, anticorps EGfr, anticorps EK2-6, anticorps Egf, anticorps Egf-r, anticorps EgfR, anticorps El, anticorps Elp, anticorps Elp-1, anticorps Elp-B1, anticorps Elp-B1RB1, anticorps HD-33, anticorps TOP, anticorps Torpedo/DER, anticorps Torpedo/Egfr, anticorps c-erbB, anticorps d-egf-r, anticorps dEGFR, anticorps dEGFR1, anticorps dEgfr, anticorps der, anticorps egfr, anticorps flb, anticorps l(2)05351, anticorps l(2)09261, anticorps l(2)57DEFa, anticorps l(2)57EFa, anticorps l(2)57Ea, anticorps mor1, anticorps top, anticorps top/DER, anticorps top/flb, anticorps torpedo/Egfr, anticorps torpedo/egfr, anticorps EGFR12, anticorps EGFR15, anticorps egfr1, anticorps Erbb2, anticorps ERBB, anticorps ERBB1, anticorps HER1, anticorps PIG61, anticorps mENA, anticorps ErbB-1, anticorps Errp, anticorps 9030024J15Rik, anticorps AI552599, anticorps Erbb, anticorps Errb1, anticorps Wa5, anticorps wa-2, anticorps wa2, anticorps epidermal growth factor receptor, anticorps Epidermal growth factor receptor, anticorps epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian), anticorps EGFR, anticorps Egfr, anticorps egfra, anticorps egfr1, anticorps LOC5564544

Sujet

Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2

ID gène

1956

UniProt

P00533

Pathways

Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins