AKT1 anticorps (AA 471-475)

N° du produit ABIN362584

Cet anticorps anti-AKT1 est un anticorps Lapin Polyclonal détectant AKT1 dans WB et IHC. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 15+ publications.

Aperçu rapide pour AKT1 anticorps (AA 471-475) (ABIN362584)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-AKT1 anticorps

Épitope: AA 471-475

Specificité: The antibody detects endogenous level of total Akt protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Peptide sequence around AA 471-475 (Q-F-S-Y-S) derived from Human Akt. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-AKT1 (ABIN362584)

Ding, Jin, Liang, Wang, Chen, Datta, Zhang, Zhang, Chen: "Transforming growth factor ? induces expression of connective tissue growth factor in hepatic progenitor cells through Smad independent signaling." dans: Cellular signalling, Vol. 25, Issue 10, pp. 1981-92, (2013) (PubMed).

Ma, Li, Wang, Gong, Zhang, Li, Ji, Liu, Chen, Xiang: "Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-Akt signaling pathways." dans: Regulatory peptides, Vol. 181, pp. 9-16, (2013) (PubMed).

Zhang, Hou, Cui, Guo, Shi, Yang, Dai: "Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity." dans: Toxicology letters, Vol. 218, Issue 1, pp. 61-9, (2013) (PubMed).

Shen, Song, An, Li, Wu, Ruan, Hu, Hu: "The use of hollow mesoporous silica nanospheres to encapsulate bortezomib and improve efficacy for non-small cell lung cancer therapy." dans: Biomaterials, Vol. 35, Issue 1, pp. 316-26, (2013) (PubMed).

Li, Bu, Wu, Wu, Huang: "The "HER2-PI3K/Akt-FASN Axis" regulated malignant phenotype of colorectal cancer cells." dans: Lipids, Vol. 47, Issue 4, pp. 403-11, (2012) (PubMed).

Luan, Zhao, Zhao, Zhu, Xiu, Ji: "Hydrogen sulfide postconditioning protects isolated rat hearts against ischemia and reperfusion injury mediated by the JAK2/STAT3 survival pathway." dans: Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], Vol. 45, Issue 10, pp. 898-905, (2012) (PubMed).

Guo, Yang, Lu, Li, Wan, Long, Li, Feng, Li: "Rb1 postconditioning attenuates liver warm ischemia-reperfusion injury through ROS-NO-HIF pathway." dans: Life sciences, Vol. 88, Issue 13-14, pp. 598-605, (2011) (PubMed).

Yu, Qin, He, Wang, Fu, Liu, Guo, Liang, Dou, Han: "Canonical notch pathway protects hepatocytes from ischemia/reperfusion injury in mice by repressing reactive oxygen species production through JAK2/STAT3 signaling." dans: Hepatology (Baltimore, Md.), Vol. 54, Issue 3, pp. 979-88, (2011) (PubMed).

Ma, Yao, Zhang, Lin, Wang, Wu, Sun, Chen: "The effects of artesunate on the expression of EGFR and ABCG2 in A549 human lung cancer cells and a xenograft model." dans: Molecules (Basel, Switzerland), Vol. 16, Issue 12, pp. 10556-69, (2011) (PubMed).

Chen, Evans, Evans: "FAM129B/MINERVA, a novel adherens junction-associated protein, suppresses apoptosis in HeLa cells." dans: The Journal of biological chemistry, Vol. 286, Issue 12, pp. 10201-9, (2011) (PubMed).

Ban, Uno, Nakamura: "Suppression of hypoxia-induced HIF-1alpha accumulation by VEGFR inhibitors: Different profiles of AAL993 versus SU5416 and KRN633." dans: Cancer letters, Vol. 296, Issue 1, pp. 17-26, (2010) (PubMed).

Nabissi, Morelli, Amantini, Farfariello, Ricci-Vitiani, Caprodossi, Arcella, Santoni, Giangaspero, De Maria, Santoni: "TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner." dans: Carcinogenesis, Vol. 31, Issue 5, pp. 794-803, (2010) (PubMed).

Toribio, Brown, Novince, Marlow, Hernon, Lanigan, Hildreth, Werbeck, Shu, Lorch, Carlton, Foley, Boyaka, McCauley, Rosol: "The midregion, nuclear localization sequence, and C terminus of PTHrP regulate skeletal development, hematopoiesis, and survival in mice." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 6, pp. 1947-57, (2010) (PubMed).

Bahk, Cho, Kim: "A cross-talk between oncogenic Ras and tumor suppressor PTEN through FAK Tyr861 phosphorylation in NIH/3T3 mouse embryonic fibroblasts." dans: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1199-204, (2008) (PubMed).

Kim, Lee, Kim, Bahk: "A Proteomic approach for protein-profiling the oncogenic ras induced transformation (H-, K-, and N-Ras) in NIH/3T3 mouse embryonic fibroblasts." dans: Proteomics, Vol. 8, Issue 15, pp. 3082-93, (2008) (PubMed).

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: Akt

Sujet: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Poids moléculaire: 60 kDa

NCBI Accession: NP_001014

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg