Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-178 amino acids from the Central region of human PRKAG3.
PRKAG3
Reactivité: Souris
WB, IHC, IF/ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Okoshi, Ohta, Ishikawa, Sato, Kizaki: "Expression of 5'-AMP-activated protein kinase with starvation in murine thymocytes." dans: The Bulletin of Tokyo Dental College, Vol. 52, Issue 1, pp. 21-9, (2011) (PubMed).
Fukuyama, Ohta, Okoshi, Suehara, Kizaki, Nakagawa: "Hypoxia induces expression and activation of AMPK in rat dental pulp cells." dans: Journal of dental research, Vol. 86, Issue 9, pp. 903-7, (2007) (PubMed).
PRKAG3 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.