IL12 anticorps

N° du produit ABIN465143

Détail du produit anti-IL12 anticorps

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL12 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Human IL-12

Purification: Protein A purified

Immunogène: CHO derived recombinant Human IL-12.

Type of Immunogen: Cell extract

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA (2 - 4 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody stained PBMC. The primary antibody was incubated at 10 μg/mL for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human IL-12 when used with biotinylated antigen affinity purified anti-human IL-12 (LS-C104425) as the detection antibody at a concentration of approximately 4-8 μg/mL. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1 ng/lane, under non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur IL12

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL12 / IL-12 (IL12 Produits)

Synonymes: anticorps CLMF, anticorps CLMF2, anticorps IL-12B, anticorps NKSF, anticorps NKSF2, anticorps p40, anticorps Il-12b, anticorps Il12p40, anticorps Il-12p40, anticorps Il12, anticorps IL-12p40, anticorps IL-12, anticorps LOC100217488, anticorps IL-12p35, anticorps Il-12a, anticorps Ll12a, anticorps p35, anticorps IL12p40, anticorps IL12B, anticorps IL-12 p35, anticorps interleukin 12B, anticorps interleukin 12A, anticorps interleukin 12b, anticorps interleukin 12a, anticorps Interleukin 12 level, anticorps IL12B, anticorps IL12A, anticorps Il12b, anticorps Il12a, anticorps IL12, anticorps il12a

Sujet: Name/Gene ID: IL-12

Synonyms: IL-12

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome