MAPKAP Kinase 5 anticorps (Internal Region)

N° du produit ABIN5687995

Détail du produit anti-MAPKAP Kinase 5 anticorps

Antigène: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPKAP Kinase 5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Purification: MK5 / MAPKAPK5 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: MK5 / MAPKAPK5 Antibody was raised against synthetic peptide STEALDNVLPSAQ found at an internal region of MK5 / MAPKAPK5.

Information d'application

Indications d'application: ELISA: antibody detection limit dilution 1:32000. Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8μ,g/mL. Immunoprecipitation:MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11927 or EB11928. Western blots of

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 500 μg/mL

Buffer: MK5 / MAPKAPK5 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C. Minimize freezing and thawing.

Détails sur MAPKAP Kinase 5

Antigène: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Autre désignation: MK5 / MAPKAPK5 (MAPKAPK5 Produits)

Synonymes: anticorps MAPKAPK5, anticorps MK5, anticorps PRAK, anticorps MAPKAP-K5, anticorps MK-5, anticorps mitogen-activated protein kinase-activated protein kinase 5, anticorps MAP kinase-activated protein kinase 5, anticorps MAPKAPK5, anticorps mapkapk5, anticorps Mapkapk5

ID gène: 8550

NCBI Accession: NP_003659, NP_620777

UniProt: Q8IW41

Pathways: Signalisation MAPK