MAPKAP Kinase 5 anticorps (Internal Region)

N° du produit ABIN5687996

Détail du produit anti-MAPKAP Kinase 5 anticorps

Antigène: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPKAP Kinase 5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Purification: MK5 / MAPKAPK5 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: MK5 / MAPKAPK5 Antibody was raised against synthetic peptide PQVLEAQRRHQKEKS found at an internal region of MK5 / MAPKAPK5.

Information d'application

Indications d'application: ELISA: antibody detection limit dilution 1:64000. Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8μ,g/mL. Immunoprecipitation:MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg EB11927 or EB11928. Western blots of

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 500 μg/mL

Buffer: MK5 / MAPKAPK5 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C. Minimize freezing and thawing.

Détails sur MAPKAP Kinase 5

Antigène: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Autre désignation: MK5 / MAPKAPK5 (MAPKAPK5 Produits)

Synonymes: anticorps MAPKAPK5, anticorps MK5, anticorps PRAK, anticorps MAPKAP-K5, anticorps MK-5, anticorps mitogen-activated protein kinase-activated protein kinase 5, anticorps MAP kinase-activated protein kinase 5, anticorps MAPKAPK5, anticorps mapkapk5, anticorps Mapkapk5

ID gène: 8550

NCBI Accession: NP_003659, NP_620777

UniProt: Q8IW41

Pathways: Signalisation MAPK