AKT1 anticorps (Internal Region)

N° du produit ABIN6391337

L’anticorps Chèvre Polyclonal anti-AKT1 a été validé pour WB et ELISA. Il convient pour détecter AKT1 dans des échantillons de Humain et Souris.

Aperçu rapide pour AKT1 anticorps (Internal Region) (ABIN6391337)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT1 est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-AKT1 anticorps

Épitope: Internal Region

Fonction: AKT1

Séquence: EVSLAKPKHR

Specificité: Variants one, two and three encode the same protein (NP_005154, NP_001014432, NP_001014431). No cross-reactivity expected with AKT2 or AKT3.

Réactivité croisée: Boeuf (Vache), Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in MCF7 and approx. 60-65 kDa in NIH3T3 cell lysates (calculated MW of 55.7 kDa according to Human NP_005154.2 and Mouse NP_033782.1). This molecular weight is routinely observed by other sources. Recommended conce

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1

Sujet: AKT1, v-akt murine thymoma viral oncogene homolog 1 , HGNC:391, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA , RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha

ID gène: 207

NCBI Accession: NP_005154, NP_001014432, NP_001014431

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg