AKT1 anticorps (AA 85-189)

N° du produit ABIN6939335

Cet anticorps anti-AKT1 Monoclonal Souris (Clone AKT1-2491) (ABIN6939335) détecte spécifiquement AKT1 dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour AKT1 anticorps (AA 85-189) (ABIN6939335)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AKT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: AKT1-2491

Détail du produit anti-AKT1 anticorps

Épitope: AA 85-189

Fonction: AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody

Specificité: Recognizes a protein of 62 kDa, which is identified as AKT1The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Positive Control: PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1

Sujet:

Oncogene AKT1, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC Alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

MCF-7 cells. PDGF-treated NIH/3T3 cells. HeLa or Human Brain, Human Kidney, Human Liver, Human Lung, Human Pancreas, Human Sk. Muscle or Human Spleen

Poids moléculaire: 62kDa

ID gène: 207, 525622

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg