HIF1A anticorps
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- Antigène Voir toutes HIF1A Anticorps
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HIF1A est non-conjugé
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Purified by Protein A/G
- Immunogène
- Recombinant human HIF1 alpha protein
- Clone
- HIF1A-84
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product HIF1A Anticorps primaire
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- Indications d'application
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Positive Control: Cobalt chloride treated HeLa cells. Breast or bladder carcinomas.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Date de péremption
- 24 months
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- Antigène
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- Autre désignation
- HIF1A (HIF1A Produits)
- Synonymes
- anticorps hif1a, anticorps AA959795, anticorps HIF1alpha, anticorps MOP1, anticorps bHLHe78, anticorps HIF-1A, anticorps HIF-1alpha, anticorps HIF1, anticorps HIF1-ALPHA, anticorps PASD8, anticorps HIF1-alpha, anticorps hif-1a, anticorps hypoxia inducible factor 1 alpha, anticorps hypoxia inducible factor 1, alpha subunit, anticorps hypoxia inducible factor 1 alpha subunit, anticorps HIF (Hypoxia Inducible Factor) homologa, anticorps hif-1a, anticorps Hif1a, anticorps HIF1A, anticorps hif1a, anticorps hif-1
- Sujet
- HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex, ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
- Poids moléculaire
- 92-110kDa
- ID gène
- 3091
- UniProt
- Q16665
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg
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