AKT1 anticorps (Internal Region)

N° du produit ABIN940546

Cet anticorps Chèvre Polyclonal détecte spécifiquement AKT1 dans WB, ELISA et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour AKT1 anticorps (Internal Region) (ABIN940546)

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AKT1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-AKT1 anticorps

Épitope: Internal Region

Fonction: AKT1

Séquence: QDVDQREAPL N

Specificité: All variants represent identical protein (NP_005154.2, NP_001014431.1, NP_001014432.1). This antibody is NOT expected to cross-react with AKT2. It may recognize AKT1 in Rat and Dog.

Réactivité croisée: Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QDVDQREAPLN, from the internal region of the protein sequence according to NP_005154.2, NP_001014431.1, NP_001014432.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in MCF7 cell lysates (calculated MW of 55.7 kDa according to NP_005154.2). This molecular weight is routinely observed by other sources. Recommended concentration:0.1-0.3 μg/mL. Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the microtubules/cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur AKT1

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT1

Sujet: AKT1, v-akt murine thymoma viral oncogene homolog 1 , AKT, MGC99656, PKB, PRKBA, RAC, RAC-ALPHA , RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, protein kinase B, rac protein kinase alpha

ID gène: 207

NCBI Accession: NP_005154, NP_001014431, NP_001014432

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg