STAT3 anticorps (Internal Region, pTyr705)

N° du produit ABIN964926

Détail du produit anti-STAT3 anticorps

Antigène: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Épitope: Internal Region, pTyr705

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificité: Anti-Stat3 antibodyis an affinity-purified antibody and is directed against the phosphorylatedform of human Stat3 protein at the Y705. Anti-Stat3 antibody was affinitypurified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizingpeptide. The resultant affinity purified antibody was then cross adsorbedagainst the non-phosphorylated form of the immunizing peptide. Anti-Stat3antibody is specific for the phosphorylated form of theprotein. Reactivity with non-phosphorylated Stat3 protein is minimal byELISA and western blot. A BLAST analysis was used to suggest crossreactivity with Stat3 antibodies from human, mouse, rat, and pig based on 100%sequence homology with the immunogen. Reactivity against homologues fromother sources is not known.

Attributs du produit: Signal transducerand activator of transcription 3 (Stat3) belongs to a family of cytoplasmictranscription factors that can be activated by phophorylation by its cell surfacereceptor. Stat3 plays a key role in many cellular processes such as cell growthand apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses toactivated FGFR1, FGFR2, FGFR3 and FGFR4.Stat3 forms a homodimer or aheterodimer with a related family member (e.g. STAT1). Activation occursthrough phosphorylation of tyrosine 705and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerizationand nuclear translocation. Serine phosphorylation is important for stableDNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 canhave a dual role in cancer, it has been found that Stat3 protein can promoteoncogenesis and have a tumor suppressor role depending upon the mutationalbackground of the tumor.

Purification: purified

Stérilité: Sterile filtered

Immunogène: This affinitypurified Stat3 antibody was prepared by repeated immunizations with aphosphorylated synthetic peptide corresponding to the region of amino acids surroundingtyrosine 705 of human Stat3.
Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application: STAT3 pY705 antibody has been tested for use in ELISA, IHC, and Western Blot. Expect a band approximately 88.1 kDa in size corresponding to phosphorylated STAT3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Commentaires:

Gene Name: STAT3

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Date de péremption: 12 months

Détails sur STAT3

Antigène: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Autre désignation: STAT3 (STAT3 Produits)

Synonymes: anticorps 1110034C02Rik, anticorps AW109958, anticorps Aprf, anticorps APRF, anticorps HIES, anticorps Xstat3, anticorps aprf, anticorps hies, anticorps stat3, anticorps wu:fc15d02, anticorps wu:fl59g06, anticorps z-Stat3, anticorps signal transducer and activator of transcription 3, anticorps signal transduction and activation of transcription 3, anticorps signal transducer and activator of transcription 3, gene 1 L homeolog, anticorps signal transducer and activator of transcription 3 (acute-phase response factor), anticorps STAT3, anticorps stat3, anticorps Stat3, anticorps stat3.1.L

Sujet: Signal transducer and activator of transcription 3 (Stat3) belongs to a family of cytoplasmic transcription factors that can be activated by phophorylation by its cell surfacereceptor. Stat3 plays a key role in many cellular processes such as cell growth and apoptosis. It also mediates cellular responses to interleukins, KITLG/SCF,EGF, IFN-alpha and other growth factors and may mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Stat3 forms a homodimer or a heterodimer with a related family member (e.g. STAT1). Activation occurs through phosphorylation of tyrosine 705 and serine 727. Phosphorylation of Stat3 at Tyr705 induces Stat3 dimerization and nuclear translocation. Serine phosphorylation is important for stable DNA-binding of Stat3 homodimers and maximal transcriptional activity. Stat3 can have a dual role in cancer, it has been found that Stat3 protein can promote oncogenesis and have a tumor suppressor role depending upon the mutational background of the tumor.
Synonyms: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, STAT3 (phospho Y705), STAT3 (pTry705)

ID gène: 6774

NCBI Accession: NP_644805

UniProt: P40763

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor