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GZMB anticorps

GZMB Reactivité: Humain, Rat WB Hôte: Souris Monoclonal 2C5-F5 unconjugated
N° du produit ABIN967291
  • Antigène Voir toutes GZMB Anticorps
    GZMB (Granzyme B (GZMB))
    Reactivité
    • 122
    • 53
    • 24
    • 10
    • 4
    • 2
    • 1
    • 1
    Humain, Rat
    Hôte
    • 78
    • 47
    • 2
    Souris
    Clonalité
    • 76
    • 51
    Monoclonal
    Conjugué
    • 78
    • 11
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GZMB est non-conjugé
    Application
    • 71
    • 42
    • 40
    • 37
    • 17
    • 17
    • 17
    • 14
    • 13
    • 9
    • 9
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Marque
    BD Pharmingen™
    Attributs du produit
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. Please refer to us for technical protocols.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Clone
    2C5-F5
    Isotype
    IgG2a
    Top Product
    Discover our top product GZMB Anticorps primaire
  • Commentaires

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Pinkoski, Waterhouse, Heibein, Wolf, Kuwana, Goldstein, Newmeyer, Bleackley, Green: "Granzyme B-mediated apoptosis proceeds predominantly through a Bcl-2-inhibitable mitochondrial pathway." dans: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 12060-7, (2001) (PubMed).

    Rotonda, Garcia-Calvo, Bull, Geissler, McKeever, Willoughby, Thornberry, Becker: "The three-dimensional structure of human granzyme B compared to caspase-3, key mediators of cell death with cleavage specificity for aspartic acid in P1." dans: Chemistry & biology, Vol. 8, Issue 4, pp. 357-68, (2001) (PubMed).

    Sharif-Askari, Alam, Rhéaume, Beresford, Scotto, Sharma, Lee, DeWolf, Nuttall, Lieberman, Sékaly: "Direct cleavage of the human DNA fragmentation factor-45 by granzyme B induces caspase-activated DNase release and DNA fragmentation." dans: The EMBO journal, Vol. 20, Issue 12, pp. 3101-13, (2001) (PubMed).

    Barry, Heibein, Pinkoski, Lee, Moyer, Green, Bleackley: "Granzyme B short-circuits the need for caspase 8 activity during granule-mediated cytotoxic T-lymphocyte killing by directly cleaving Bid." dans: Molecular and cellular biology, Vol. 20, Issue 11, pp. 3781-94, (2000) (PubMed).

    Beresford, Xia, Greenberg, Lieberman: "Granzyme A loading induces rapid cytolysis and a novel form of DNA damage independently of caspase activation." dans: Immunity, Vol. 10, Issue 5, pp. 585-94, (1999) (PubMed).

    Shresta, Graubert, Thomas, Raptis, Ley: "Granzyme A initiates an alternative pathway for granule-mediated apoptosis." dans: Immunity, Vol. 10, Issue 5, pp. 595-605, (1999) (PubMed).

    Andrade, Roy, Nicholson, Thornberry, Rosen, Casciola-Rosen: "Granzyme B directly and efficiently cleaves several downstream caspase substrates: implications for CTL-induced apoptosis." dans: Immunity, Vol. 8, Issue 4, pp. 451-60, (1998) (PubMed).

  • Antigène
    GZMB (Granzyme B (GZMB))
    Autre désignation
    Granzyme B (GZMB Produits)
    Synonymes
    anticorps CCPI, anticorps CGL-1, anticorps CGL1, anticorps CSP-B, anticorps CSPB, anticorps CTLA1, anticorps CTSGL1, anticorps HLP, anticorps SECT, anticorps granzyme B, anticorps GZMB
    Sujet
    The primary mechanism by which cytotoxic T cells eliminate virally infected cells is by granule exocytosis. The release of cytotoxic granule contents by cytotoxic T lymphocytes (CTL) triggers apoptotic target cell death. CTL granules contain a poreforming protein, perforin, and a group of serine proteases called granzymes. In the classic model, perforins create holes in the target cell membrane, allowing entrance of the granzymes. Granzyme A and B are the predominant granzymes activated after CTL activation, but each act via an independent apoptotic pathway, granzyme B is activated immediately, while granzyme A acts hours later. The physiological substrates for granzyme A in the apoptotic pathway have not been identified. Studies involving mice which are deficient in both granzyme A and B suggest a model whereby the granzyme B pathway may have evolved as the major apoptotic pathway with the granzyme A pathway acting as a backup. Granzyme B has been shown to induce apoptosis and to cleave a number of substrates which are similar in specificity to those of the caspase family of proteinases. Granzyme B can cleave substrates, such as DNA-PKcs, and nuclear mitotic apparatus protein (NuMA). Furthermore, Granzyme B can also cleave substrates such as Bid and DFF45 in a caspase-independent fashion. However, further research is needed to delineate the exact role of caspases in cytotoxic T lymphocyte-induced apoptosis involving Granzyme B. Granzyme B migrates at approximately 32 kDa in SDS/PAGE. Clone 2C5/F5 recognizes human and rat granzyme B.
    Poids moléculaire
    32 kDa
    Pathways
    Apoptose, Caspase Cascade in Apoptosis
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