AKT1 Kit ELISA

N° du produit ABIN1571880

Détail du produit AKT1 Kit ELISA

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PKBa in human tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.053 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: Protein Kinase B Alpha (PKBa) (AKT1 Produits)

Synonymes: AKT Kit ELISA, CWS6 Kit ELISA, PKB Kit ELISA, PKB-ALPHA Kit ELISA, PRKBA Kit ELISA, RAC Kit ELISA, RAC-ALPHA Kit ELISA, Akt Kit ELISA, Rac Kit ELISA, PKB/Akt Kit ELISA, PKBalpha Kit ELISA, AKT/PKB Kit ELISA, AKT1 Kit ELISA, Akt/PKB Kit ELISA, Akt1 Kit ELISA, CG4006 Kit ELISA, D-Akt Kit ELISA, DAKT1 Kit ELISA, DAKT1/PKB Kit ELISA, DAkt Kit ELISA, DAkt1 Kit ELISA, DPKB Kit ELISA, DRAC-PK Kit ELISA, DRAC-PK66 Kit ELISA, DRAC-PK85 Kit ELISA, Dakt Kit ELISA, Dakt1 Kit ELISA, Dmel\\CG4006 Kit ELISA, Dpkb Kit ELISA, PKB/AKT Kit ELISA, PKB/dAKT Kit ELISA, RacPK Kit ELISA, akt Kit ELISA, akt1 Kit ELISA, dAKT Kit ELISA, dAKT/dPKB Kit ELISA, dAKT1 Kit ELISA, dAkt Kit ELISA, dAkt/PKB Kit ELISA, dAkt1 Kit ELISA, dPKB Kit ELISA, dakt Kit ELISA, dakt1 Kit ELISA, l(3)04226 Kit ELISA, l(3)89Bq Kit ELISA, p-Akt Kit ELISA, pAkt Kit ELISA, ACT-5 Kit ELISA, akt-1 Kit ELISA, pkb Kit ELISA, v-akt Kit ELISA, v-akt1 Kit ELISA, xAct Kit ELISA, ATAKT1 Kit ELISA, F18A8.2 Kit ELISA, F18A8_2 Kit ELISA, K+ transporter 1 Kit ELISA, POTASSIUM TRANSPORTER Kit ELISA, AKT serine/threonine kinase 1 Kit ELISA, thymoma viral proto-oncogene 1 Kit ELISA, CG4006 gene product from transcript CG4006-RE Kit ELISA, actin beta Kit ELISA, v-akt murine thymoma viral oncogene homolog 1 S homeolog Kit ELISA, v-akt murine thymoma viral oncogene homolog 1 Kit ELISA, K+ transporter 1 Kit ELISA, Serine/threonine-protein kinase akt-1 Kit ELISA, AKT1 Kit ELISA, Akt1 Kit ELISA, ACTB Kit ELISA, akt1.S Kit ELISA, akt1 Kit ELISA, KT1 Kit ELISA, akt-1 Kit ELISA

Sujet: Alternative name: AKT1, AKT, PKB-A, Proto-oncogene c-Akt, RAC-Alpha Serine/Threonine-Protein Kinase, V-akt Murine Thymoma Viral Oncogene Homolog 1

ID gène: 207

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg