CCL2 Kit ELISA
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- Antigène Voir toutes CCL2 Kits ELISA
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Rat MCP-1 (CCL2) SpeedELISA Kit.
- Marque
- SpeedELISA
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Attributs du produit
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- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 μL of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 μL of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
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May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles. - Date de péremption
- 6 months
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- Antigène Voir toutes CCL2 Kits ELISA
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Autre désignation
- MCP-1 (CCL2 Produits)
- Synonymes
- GDCF-2 Kit ELISA, HC11 Kit ELISA, HSMCR30 Kit ELISA, MCAF Kit ELISA, MCP-1 Kit ELISA, MCP1 Kit ELISA, SCYA2 Kit ELISA, SMC-CF Kit ELISA, AI323594 Kit ELISA, JE Kit ELISA, Scya2 Kit ELISA, Sigje Kit ELISA, MCP-1A Kit ELISA, MCP1A Kit ELISA, C-C motif chemokine ligand 2 Kit ELISA, chemokine (C-C motif) ligand 2 Kit ELISA, C-C motif chemokine 2 Kit ELISA, CCL2 Kit ELISA, Ccl2 Kit ELISA, LOC101120093 Kit ELISA
- ID gène
- 24770
- UniProt
- P14844
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2
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