AKT1 Kit ELISA

N° du produit ABIN4885966

Détail du produit AKT1 Kit ELISA

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Épitope: pSer473

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Phospho-AKT/GSKß/mTor ELISA Kit. This assay semi-quantitatively measures phophorylated AKT/GSK-beta/mTor in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes phosphorylated AKT (S473), GSK3? (S9), mTOR (S2448), and total AKT, GSK3?, mTOR in Human Cell Lysates.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Autre désignation: AKT (AKT1 Produits)

Synonymes: AKT Kit ELISA, CWS6 Kit ELISA, PKB Kit ELISA, PKB-ALPHA Kit ELISA, PRKBA Kit ELISA, RAC Kit ELISA, RAC-ALPHA Kit ELISA, Akt Kit ELISA, Rac Kit ELISA, PKB/Akt Kit ELISA, PKBalpha Kit ELISA, AKT/PKB Kit ELISA, AKT1 Kit ELISA, Akt/PKB Kit ELISA, Akt1 Kit ELISA, CG4006 Kit ELISA, D-Akt Kit ELISA, DAKT1 Kit ELISA, DAKT1/PKB Kit ELISA, DAkt Kit ELISA, DAkt1 Kit ELISA, DPKB Kit ELISA, DRAC-PK Kit ELISA, DRAC-PK66 Kit ELISA, DRAC-PK85 Kit ELISA, Dakt Kit ELISA, Dakt1 Kit ELISA, Dmel\\CG4006 Kit ELISA, Dpkb Kit ELISA, PKB/AKT Kit ELISA, PKB/dAKT Kit ELISA, RacPK Kit ELISA, akt Kit ELISA, akt1 Kit ELISA, dAKT Kit ELISA, dAKT/dPKB Kit ELISA, dAKT1 Kit ELISA, dAkt Kit ELISA, dAkt/PKB Kit ELISA, dAkt1 Kit ELISA, dPKB Kit ELISA, dakt Kit ELISA, dakt1 Kit ELISA, l(3)04226 Kit ELISA, l(3)89Bq Kit ELISA, p-Akt Kit ELISA, pAkt Kit ELISA, ACT-5 Kit ELISA, akt-1 Kit ELISA, pkb Kit ELISA, v-akt Kit ELISA, v-akt1 Kit ELISA, xAct Kit ELISA, ATAKT1 Kit ELISA, F18A8.2 Kit ELISA, F18A8_2 Kit ELISA, K+ transporter 1 Kit ELISA, POTASSIUM TRANSPORTER Kit ELISA, AKT serine/threonine kinase 1 Kit ELISA, thymoma viral proto-oncogene 1 Kit ELISA, CG4006 gene product from transcript CG4006-RE Kit ELISA, actin beta Kit ELISA, v-akt murine thymoma viral oncogene homolog 1 S homeolog Kit ELISA, v-akt murine thymoma viral oncogene homolog 1 Kit ELISA, K+ transporter 1 Kit ELISA, Serine/threonine-protein kinase akt-1 Kit ELISA, AKT1 Kit ELISA, Akt1 Kit ELISA, ACTB Kit ELISA, akt1.S Kit ELISA, akt1 Kit ELISA, KT1 Kit ELISA, akt-1 Kit ELISA

ID gène: 391

UniProt: P31749

Pathways: Signalisation PI3K-Akt, Signalisation RTK, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, L'effet Warburg