PPP3R1 Kit ELISA

N° du produit ABIN5674873

Détail du produit PPP3R1 Kit ELISA

Antigène: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Épitope: Regulatory Subunit 1

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 15.5 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Autre désignation: Protein Phosphatase 3, (PPP3R1) (PPP3R1 Produits)

Synonymes: si:ch211-274c22.8 Kit ELISA, canb Kit ELISA, CALNB1 Kit ELISA, CNB Kit ELISA, CNB1 Kit ELISA, CaNB1 Kit ELISA, Cnb1 Kit ELISA, MCIP1 Kit ELISA, protein phosphatase 3, regulatory subunit B, alpha a Kit ELISA, protein phosphatase 3 regulatory subunit B, alpha S homeolog Kit ELISA, protein phosphatase 3 regulatory subunit B, alpha Kit ELISA, protein phosphatase 3, regulatory subunit B, alpha Kit ELISA, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) Kit ELISA, ppp3r1a Kit ELISA, ppp3r1.S Kit ELISA, PPP3R1 Kit ELISA, Ppp3r1 Kit ELISA

Sujet: Alternative name: CALNB1, CNB, CNB1, Calcineurin B Type I, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

ID gène: 5534

UniProt: P63098

Pathways: Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling