IL-1 beta Kit ELISA

N° du produit ABIN7066375

Détail du produit IL-1 beta Kit ELISA

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1b in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b)

Réactivité croisée (Details): No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

Sensibilité: 6.1 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 0.5mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 1,000pg/mL. Prepare 7 tubes containing 0.25mL Standard Diluent and produce a double dilution series Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Stock: 4 °C/-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Date de péremption: 6 months

Références pour IL-1 beta Kit ELISA (ABIN7066375)

Jackson, Reppe, Eidet, Eide, Tønseth, Bergersen, Dartt, Griffith, Utheim: "Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets." dans: Scientific reports, Vol. 7, Issue 1, pp. 8206, (2019) (PubMed).

Song, Park, Lee, Lee: "Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage." dans: The journal of maternal-fetal & neonatal medicine, Vol. 32, Issue 14, pp. 2287-2294, (2019) (PubMed).

Zhang, Zhang, Xu, Yin, Huang, Wang, Zhang, Huang, Li: "Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro." dans: International immunopharmacology, Vol. 49, pp. 222-230, (2018) (PubMed).

Ma, An, Wang: "Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy." dans: Endocrine, metabolic & immune disorders drug targets, Vol. 17, Issue 2, pp. 114-124, (2018) (PubMed).

Cen, Liu, Wang, Yang, Shi, Liang: "Glucosamine oral administration as an adjunct to hyaluronic acid injection in treating temporomandibular joint osteoarthritis." dans: Oral diseases, Vol. 24, Issue 3, pp. 404-411, (2018) (PubMed).

Wu, Hong, Wu, Wang, Chen, Wang, Cheng, Xia: "Stromal-epithelial lactate shuttle induced by tumor‑derived interleukin‑1β promotes cell proliferation in oral squamous cell carcinoma." dans: International journal of molecular medicine, Vol. 41, Issue 2, pp. 687-696, (2018) (PubMed).

Mancini, Milardi, Carfagna, Grande, Miranda, De Cicco Nardone, Ricciardi, Pontecorvi, Marana, De Cicco Nardone: "Low-dose SKA Progesterone and Interleukin-10 modulate the inflammatory pathway in endometriotic cell lines." dans: International immunopharmacology, Vol. 55, pp. 223-230, (2018) (PubMed).

Štofilová, Langerholc, Botta, Treven, Gradišnik, Salaj, Šoltésová, Bertková, Hertelyová, Bomba: "Cytokine production in vitro and in rat model of colitis in response to Lactobacillus plantarum LS/07." dans: Biomedicine & pharmacotherapy, Vol. 94, pp. 1176-1185, (2018) (PubMed).

Zhou, Ge, Ding, Qu, Wei, Wu, Yao, Wei, Feng, Long, Deng: "Relationship between serum adipsin and the first phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance." dans: Journal of diabetes investigation, Vol. 9, Issue 5, pp. 1128-1134, (2018) (PubMed).

Luo, Wang, Liu, Yin, A, Huang, Ye: "Inhibition of SOX9 Promotes Inflammatory and Immune Responses of Dental Pulp." dans: Journal of endodontics, Vol. 44, Issue 5, pp. 792-799, (2018) (PubMed).

Tersigni, DIppolito, Di Nicuolo, Marana, Valenza, Masciullo, Scaldaferri, Malatacca, de Waure, Gasbarrini, Scambia, Di Simone: "Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?" dans: Journal of translational medicine, Vol. 16, Issue 1, pp. 102, (2018) (PubMed).

Gong, Guan, Tian, Li, Zhang: "Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 3514-3523, (2018) (PubMed).

Mern, Tschugg, Hartmann, Thomé et al.: "Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new ..." dans: PLoS ONE, Vol. 12, Issue 2, pp. e0172181, (2017) (PubMed).

Abduljabbar, Vohra, Kellesarian, Javed et al.: "Efficacy of scaling and root planning with and without adjunct Nd:YAG laser therapy on clinical periodontal parameters and gingival crevicular fluid interleukin 1-beta and tumor necrosis factor-alpha ..." dans: Journal of photochemistry and photobiology. B, Biology, Vol. 169, pp. 70-74, (2017) (PubMed).

Pagani, Borsari, Veronesi, Ferrari, Cepollaro, Torricelli, Filardo, Fini: "Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow-Derived Cells in Osteoarthritic In Vitro Models." dans: Journal of cellular physiology, Vol. 232, Issue 6, pp. 1478-1488, (2017) (PubMed).

Yoshizaki, Brito, Silva, Lino-Dos-Santos-Franco, Frias, E Silva, Amato-Lourenço, Saldiva, de Fátima Lopes Calvo Tibério, Mauad, Macchione: "The effects of particulate matter on inflammation of respiratory system: Differences between male and female." dans: The Science of the total environment, Vol. 586, pp. 284-295, (2017) (PubMed).

Zhong, Liu, Zhang, Zhang, Liu, Hu: "Alterations in gut microbiota during remission and recurrence of diabetes after duodenal-jejunal bypass in rats." dans: World journal of gastroenterology, Vol. 22, Issue 29, pp. 6706-15, (2016) (PubMed).

Tian, Peng, Zhong, Yang, Pang, Lou, Li, An, Zhang, Xu, Dong: "β-Caryophyllene protects in vitro neurovascular unit against oxygen-glucose deprivation and re-oxygenation-induced injury." dans: Journal of neurochemistry, Vol. 139, Issue 5, pp. 757-768, (2016) (PubMed).

Kumar, Kumar, Gupta: "Manganese- and cobalt-based coordination networks as promising heterogeneous catalysts for olefin epoxidation reactions." dans: Inorganic chemistry, Vol. 54, Issue 6, pp. 2603-15, (2015) (PubMed).

Louzada, Araújo, Verma, Daolio, Herrmann, Tomassini: "Critical cooperation range to improve spatial network robustness." dans: PLoS ONE, Vol. 10, Issue 3, pp. e0118635, (2015) (PubMed).

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin 1 Beta (IL1b) (IL1B Produits)

Synonymes: IL-1 Kit ELISA, IL1-BETA Kit ELISA, IL1F2 Kit ELISA, IL-1BETA Kit ELISA, IL1beta Kit ELISA, il1-b Kit ELISA, zgc:111873 Kit ELISA, IL-1B Kit ELISA, IL-1beta Kit ELISA, Il-1b Kit ELISA, IL1B Kit ELISA, IL-1 beta Kit ELISA, IL-1b Kit ELISA, interleukin 1 beta Kit ELISA, interleukin 1, beta Kit ELISA, IL1B Kit ELISA, il1b Kit ELISA, Il1b Kit ELISA

UniProt: P01584

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome