CD40 Ligand Protein (CD40LG) (AA 18-111) (DYKDDDDK Tag)

N° du produit ABIN1344287

Détail du produit

Antigène: CD40 Ligand (CD40LG)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 18-111

Origine: Humain, Souris, Rat

Source: CHO Cells

Purification/Conjugué: Cette CD40 Ligand protéine est marqué à la DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Marque: MegaCD40L™

Specificité: Binds to rat, human and mouse CD40.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Attributs du produit: Rat CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Pureté: >95 % (SDS-PAGE)

niveau d'endotoxine: <0.02EU/μg purified protein (LAL test, Lonza).

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CD40 Ligand (CD40LG)

Autre désignation: MegaCD40L (CD40LG Produits)

Synonymes: CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein

Sujet: MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has been shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

Poids moléculaire: ~35-40kDa

UniProt: Q9Z2V2

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints